Part:BBa_K3782010:Design

T7-RBS-His-DcAFP-Term

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1082
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 357
  Illegal BglII site found at 425
  Illegal BglII site found at 902
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 911
  

Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.

Source

The source of DcAFP is Daucus carota. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/1139877302